Anammox Bacteria 101
There are different microbial
communities in the Anoxic Biocenosis Clarification Baskets - most people just
call it Biocenosis Baskets for a shorter name recognition - (hence the name-
biocenosis. Syd does explain about biocenosis in his articles) and the filter
containment pond that make up the biological filter and its porewater
compartments were examined to determine if the presence and/or the activity of
anaerobic ammonium-oxidizing bacterium known as Anammox bacteria were present.
Community DNA was extracted from aerobic and anaerobic fixed-film in the Anoxic
filters substrate, mulm or detritus waste collection filter compartment and was
analyzed by amplifying 16S rRNA genes by PVR using anammox-selective and universal
GC-clamped primers. Separation of amplified PCR products by denaturing gradient
gel electrophoresis and sequencing of the different phylotypes revealed a
diverse biofilter microbial community in the sludge. While Planctomycetales was
found in the microbial waste sludge communities that settles in the Anoxic
filter itself, it was not found in the Biocenosis Baskets that are committed to
the Anoxic Filtration and its biological activities. The anaerobic denitrifies
that are found in the biofilter containment department, the anaerobic
denitrifying biofilms did contain one clone that exhibited high levels of
sequence similarity known as anammox Planctomycetales bacteria. In this
biological process of reduction, nitrite with ammonium ions are converted directly
into a dinitrogen N2 gas. Fifty percent of all N2 is produced in the oceans by
anammox biofilms. The catabolic reaction is known as NH4+NO2-
N2 + 2H2O and is a fixed nitrogen. Fluorescence in situ hybridization studies
that were conducted of Anammox Planctomycetales using an anammox-specific probe
from the university confirmed the presence of Planctomycetales in the microbial
waste or mulm in the biofilms showed their activity and was detected by the use
of their ability to simultaneously consume ammonia and nitrite as stated in my
book to produce N2. To my knowledge, this is the first pond filtration system
that the identification of anammox-related sequences to take place in a closed
recirculating pond filtration system. The findings do provide a foundation for
incorporating this important biological pathway for a complete or
partial nitrogen removal in such close systems. This indicates that anammox
bacteria could be mediating dissimilatory nitrate reduction to ammonium in
natural ecosystems.
Anammox Bacteria in anoxic conditions can be part of the nitrification process in denitrification. |
Anoxic Filtration Book... Still free
on Apple's iBook store
https://itunes.apple.com/us/book/ano...04698627?mt=11
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