Wednesday, July 3, 2013

There are different microbial communities in the Anoxic Biocenosis Clarification Baskets , like Anammox Bacteria 101

Anammox Bacteria 101

There are different microbial communities in the Anoxic Biocenosis Clarification Baskets - most people just call it Biocenosis Baskets for a shorter name recognition - (hence the name- biocenosis. Syd does explain about biocenosis in his articles) and the filter containment pond that make up the biological filter and its porewater compartments were examined to determine if the presence and/or the activity of anaerobic ammonium-oxidizing bacterium known as Anammox bacteria were present. Community DNA was extracted from aerobic and anaerobic fixed-film in the Anoxic filters substrate, mulm or detritus waste collection filter compartment and was analyzed by amplifying 16S rRNA genes by PVR using anammox-selective and universal GC-clamped primers. Separation of amplified PCR products by denaturing gradient gel electrophoresis and sequencing of the different phylotypes revealed a diverse biofilter microbial community in the sludge. While Planctomycetales was found in the microbial waste sludge communities that settles in the Anoxic filter itself, it was not found in the Biocenosis Baskets that are committed to the Anoxic Filtration and its biological activities. The anaerobic denitrifies that are found in the biofilter containment department, the anaerobic denitrifying biofilms did contain one clone that exhibited high levels of sequence similarity known as anammox Planctomycetales bacteria. In this biological process of reduction, nitrite with ammonium ions are converted directly into a dinitrogen N2 gas. Fifty percent of all N2 is produced in the oceans by anammox biofilms. The catabolic reaction is known as NH4+NO2-  N2 + 2H2O and is a fixed nitrogen.  Fluorescence in situ hybridization studies that were conducted of Anammox Planctomycetales using an anammox-specific probe from the university confirmed the presence of Planctomycetales in the microbial waste or mulm in the biofilms showed their activity and was detected by the use of their ability to simultaneously consume ammonia and nitrite as stated in my book to produce N2. To my knowledge, this is the first pond filtration system that the identification of anammox-related sequences to take place in a closed recirculating pond filtration system. The findings do provide a foundation for incorporating this important biological pathway for a complete or partial nitrogen removal in such close systems. This indicates that anammox bacteria could be mediating dissimilatory nitrate reduction to ammonium in natural ecosystems.
Anammox Bacteria in anoxic conditions can be part of the nitrification process in denitrification.

Anoxic Filtration Book... Still free on Apple's iBook store

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